Instrumentation

The MacIsaac Facility core equipment ranges in both age and complexity, however, a unique variety of instrumentation is available for both external and internal use.

  • Becton Dickinson (BD) FACScan benchtop analyzer - currently the oldest instrument within the Facility (purchased 1991), however, major upgrades to both the laser (solid-state, Blue – 488 nm 2010 & 2015) and the computer system have continued to maintain this instrument and its capabilities.
  • Beckman Coulter (formerly Cytomation) Legacy MoFlo – purchased in 1999, this instrument was the replacement cell-sorter for the Facility’s former BC EPICS V instrument. This instrument is used primarily for its unique ability to sort large particles (>40 µm). The sorter has had one major upgrade in 2011 (new primary 488 nm solid state laser). [Legacy MoFlo] (IMG_0288.jpeg)
  • BD (formerly Cytopeia) Influx Mariner – purchased in 2006, and initially housed within a Sea-going Cell Sorting Laboratory (UNOLS laboratory van), acquired using a 2005 NSF MRI grant. This instrument is currently located within the SCGC Facility clean room and is the primary cell-sorter used for single-cell genomics sorting and most bulk sorting needs of the Facility. It has 3 solid-state lasers (Blue – 488 nm, UV – 365 nm, and Red – 635 nm), two lasers are currently in use (Blue and UV). [Influx Mariner] (IMG_0292.jpeg)
  • BD (formerly Cytopeia) Influx Mariner – includes an additional UNOLS certified laboratory van. The instrument is currently housed within the laboratory van and is accessible from the basement of the Center for Blue Biotechnology. It is maintained by the Facility and is primarily used by the Lomas Lab as-fee-for-service. The instrument has 3 solid-state lasers.
  • BD FACS Jazz/Influx Mariner –The instrument is maintained by the Facility and has 3 fully functional solid-state lasers (Blue – 488 nm, UV – 365 nm, and Red – 635 nm).
  • Fluid Imaging Technologies FlowCAM – acquired in 2004, one of the original FlowCAM instruments, has had two computer upgrades (2007, 2013), two laser replacements (2007, 2013), and one hardware replacement – digital processor (2007).
  • Zeiss Standard Fluorescence Microscope and a Diagnostics Instruments Spot RT-KE Slider color camera with an automated stage. This is one of two imaging systems maintained by the Facility.
  • Olympus Inverted Fluorescence Microscope and a 1Kx1K Photometrics (Roper Scientific) cooled, CCD camera.