Vishaan Nursey

Research Technician
Phone: +1 (207) 315-2567, ext. 531
vnursey@bigelow.org

For media inquiries, please contact sprofaizer@bigelow.org


BioPublicationsCV

Lab PageLab Page

Education

B.S., Natural Sciences with concentrations in Neuroscience and Public Health, Johns Hopkins University, 2018

Research Interests

I am a Benchtop generalist working primarily on devising methods to reduce methane emissions from ruminating livestock. To this end, I utilize a variety of techniques including, gene editing, culturing of various genetically modified yeast and algae strains, and protein purification as well as characterization. I am interested in the potential of heterologous expression of proteins and other bioactive compounds found in marine organisms to improve and revolutionize the agricultural and other large-scale industries.

Cell Culturing
  • Growth and maintenance of various yeast and microalgal cell lines.
  • Large scale yeast cultivation operations utilizing 10L bioreactor.
  • Media prep
  • Aseptic technique

Molecular biology assays and techniques
  • Large- and small-scale cell lysis using a variety of physical methods
  • PCR proficiency and experience with primer development
  • Mini preps and DNA isolations
  • PCR clean-ups
  • Gibson Assembly and other recombinant expression techniques.
  • ELISA’s, Bradford and other plate-based assays
  • Protein and DNA visualization using SDS-PAGE and TAE agarose

Analytical techniques/instrument experience
  • Plate reader
  • Flourometer
  • UV/VIS Spectrophotometer
  • Protein purification using fast protein liquid chromatography (FPLC)

Publications

2023

  • Heaney, C., Knisel, A., Vuthoori, R., Golombeck, D., Fernandez, H., Lima, B., Taylor, J., Davidson, K., Kennedy, K., Nursey, V., Miller, E., & Maybaum, S. (2023). Subjective Assessment Underestimates Fraility in Patients With Heart Failure Referred for Advanced Therapies. ASAIO journal (American Society for Artificial Internal Organs : 1992), e001894. Advance online publication. https://doi.org/10.1097/MAT.0000000000001894

2022

  • Moldwin, R. M*., Nursey, V.*, Yaskiv, O., Dalvi, S., Macdonald, E. J., Funaro, M., Zhang, C., DeGouveia, W., Ruzimovsky, M., Rilo, H. R., Miller, E. J., Najjar, S., Tabansky, I., & Stern, J. N. H. (2022). Immune cell profiles of patients with interstitial cystitis/bladder pain syndrome. Journal of translational medicine, 20(1), 97. https://doi.org/10.1186/s12967-022-03236-7 * denotes co-first authorship.
  • Tabansky, I., Moldwin, R. M., Liu, M., Najjar, S., Keskin, D. B., Nursey, V., Laurent, M., Birder, L. A., Brusic, V., Zhang, G., & Stern, J. N. H. (2022). A shared B-cell clonotype in patients with interstitial cystitis/bladder pain syndrome presenting with Hunner lesions. Continence Reports, 4, 100015. https://doi.org/10.1016/j.contre.2022.100015
  • Tabansky, I., Tanaka, A. J., Wang, J., Zhang, G., Dujmovic, I., Mader, S., Jeganathan, V., DeAngelis, T., Funaro, M., Harel, A., Messina, M., Shabbir, M., Nursey, V., DeGouvia, W., Laurent, M., Blitz, K., Jindra, P., Gudesblatt, M., Regeneron Genetics Center, King, A., … Stern, J. N. H. (2022). Rare variants and HLA haplotypes associated in patients with neuromyelitis optica spectrum disorders. Frontiers in immunology, 13, 900605. https://doi.org/10.3389/fimmu.2022.900605

2019

  • Harel, A., Nursey, V., Stern, J., & Najjar, S. (2019). Development of Resistance to B-Cell Depletion Following Ten Years of Effective Rituximab Treatment in a Patient with Neuromyelitis Optica (P1. 2-008).

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