SCGC marks 5th year with new services

12-08-2014

SCGC has made significant progress scaling up and reducing the cost of single cell genomic sequencing services. Scaling up was achieved with new instrumentation made possible by the National Science Foundation and Illumina. The new techniques created an eightfold reduction in costs and expedited turnaround times.

The small but persistent cross-talk of multiplexed libraries, produced by standard next-gen sequencing techniques (1-4), was a major challenge in single cell genomics, which relies on de novo assembly of deeply sequenced MDA products with highly uneven sequence coverage (5-9). For this reason, SCGC was processing one single amplified genome (SAG) per sequencing run (no multiplexing), resulting in contamination-free assemblies but relatively expensive and slow services. Recently, SCGC's acquisition of new instrumentation, sponsored by NSF and Illumina grants, and extensive R&D efforts by the SCGC team have allowed us to eliminate the cross-talk when analyzing multiplexed libraries, permitting clean, fast and cost-effective SAG genomic sequencing. Specifically, this has enabled an 8-fold cost reduction for large WGS projects and the completion of expedited services in 10 business days.

Descriptions of new genomic sequencing services with associated fees and benchmark data are available on the [140]SCGC website.

SCGC is delighted to be celebrating its fifth anniversary and is looking forward to continuing to serve as an engine for continued discoveries. The SCGC Team: Front from left: Drs. Nicole Poulton, Ramunas Stepanauskas Back from left: Brian Thompson, Liz Fergusson, Ben Tupper, Corianna Mascena, Joe Brown, Jessica Labonte