SCGC-enabled research publications
2013
- Lloyd KG, Schreiber L, Petersen DG, Kjeldsen K, Lever MA, Stepanauskas R, Richter M, Kleindienst S, Lenk S, Schramm A, Jorgensen BB. 2013. Predominant archaea in marine sediments degrade detrital proteins. Nature 496:215-218.
- Zhaxybayeva O, Stepanauskas R, Mohan NR, Papke RT. 2013. Cell sorting analysis of geographically separated hypersaline environments. Extremophiles 17:265-75.
- Garcia LG, McMahon KD, Martinez-Garcia M, Srivastava A, Sczyrba A, Stepanauskas R, Grossart HP, Woyke T, Warnecke F. 2013. Metabolic potential of a single cell belonging to one of the most abundant lineages in freshwater bacterioplankton. The ISME Journal 7:137–147.
2012
- Stepanauskas, R. 2012. Single cell genomics: an individual look at microbes. Current Opinion in Microbiology, 15:613–620.
- Martinez-Garcia M, Brazel DM, Swan BK, Arnosti C, Chain PSG, Reitenga KG, Xie G, Poulton NJ, Lluesma Gomez M, Masland DED, Thompson B, Bellows WK, Ziervogel K, Lo CC, Ahmed S, Gleasner CD, Detter CJ, Stepanauskas R. 2012. Capturing single cell genomes of active polysaccharide degraders: An unexpected contribution of Verrucomicrobia. PLoS ONE 7:e35314.
- Martinez-Garcia M, Brazel DM, Poulton N, Swan BK, Lluesma Gomez M, Masland D, Sieracki ME, Stepanauskas R. 2012. Unveiling in situ interactions between marine protists and bacteria through single cell sequencing. The ISME Journal 6:703-707.
- Martinez-Garcia M, Swan BK, Poulton NJ, Lluesma Gomez M, Masland D, Sieracki ME, Stepanauskas R. 2012. High throughput single cell sequencing identifies photoheterotrophs and chemoautotrophs in freshwater bacterioplankton. The ISME Journal 6:113-123.
2011
- Swan BK, Martinez-Garcia M, Preston CM, Sczyrba A, Woyke T, Lamy D, Reinthaler T, Poulton NJ, Masland D, Lluesma Gomez M, Sieracki ME, DeLong EF, Herndl GJ, Stepanauskas R. 2011. Potential for chemolithoautotrophy among ubiquitous bacteria lineages in the dark ocean. Science 333:1296-1300
- Yoon HS, Price DC, Stepanauskas R, Rajah VD, Sieracki ME, Wilson WH, Yang EC, Duffy S, Bhattacharya D. 2011 Single-cell genomics reveals organismal interactions in uncultivated marine protists. Science 332:714-717
- Hess M, Sczyrba A, Egan R, Kim TW, Chokhawala H, Schroth G, Luo S, Clark DS, Chen F, Zhang T, Mackie RI, Pennacchio LA, Tringe SG, Visel A, Woyke T, Wang Z, Rubin EM. 2011. Metagenomic discovery of biomass-degrading genes and genomes from cow rumen. Science 331:463-467
- Heywood JL, Sieracki ME, Bellows W, Poulton NJ, Stepanauskas R. 2011. Capturing diversity of marine heterotrophic protists: one cell at a time. The ISME Journal 5:674-684
- Woyke T, Sczyrba A, Lee J, Rinke C, Tighe D, Clingenpeel S, Malmstrom R, Stepanauskas R, Cheng J-F. 2011. Contamination-free single cell amplification. PLoS ONE 10:e26161
- Martinez-Martinez J, Poulton N, Stepanauskas R, Sieracki M, Wilson WH (2011) Targeted Sorting of Single Virus-Infected Cells of the Coccolithophore Emiliania huxleyi. PLOS ONE 6:e22520
- Fleming EJ, Langdon AE, Martinez-Garcia M, Stepanauskas R, Poulton N, Masland D, Emerson D. 2011. What's new is old: resolving the identity of Leptothrix ochracea using single cell genomics, pyrosequencing and FISH. PLoS ONE 6:e17769
- Ghai R, Pasic L, Fernandez AB, Martin-Cuadrado AB, Mizuno CM, McMahon KD, Papke RT, Stepanauskas R, Rodriguez-Brito B, Rohwer F, Sanchez-Porro C, Ventosa A, Rodriguez-Valera F. 2011. New abundant microbial groups in aquatic hypersaline environments. Scientific Reports 1:srep00135
- Reyes-Prieto A, Yoon HS, Moustafa A, Yang EC, Andersen RA, Boo SM, Nakayama T, Ishida KI, Bhattacharya D. 2010. Differential gene retention in plastids of common recent origin. Molecular biology and evolution 27:1530-1537
Early method development publications by the SCGC team
- Stepanauskas R, Sieracki ME. 2007. Matching phylogeny and metabolism in the uncultured marine bacteria, one cell at a time. Proceedings of the National Academy of Sciences of the United States of America 104:9052-9057
- Ishoey T, Woyke T, Stepanauskas R, Novotny M, Lasken RS. 2008. Genomic sequencing of single microbial cells from environmental samples. Current Opinion in Microbiology 11:198-204
- Woyke T, Xie G, Copeland A, Gonzalez JM, Han C, Kiss H, Saw J, Senin P, Yang C, Chatterji S, Cheng J-F, Eisen JA, Sieracki ME, Stepanauskas R. 2009. Assembling the marine metagenome, one cell at a time. PLoS ONE 4:e5299
News coverage of SCGC activities
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Field j (2012) Probing Microbes of the Deep: Maine Science Lab Breaks New Ground . MPBN, November 2012 |

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Owens B (2012) Genomics: The single life. Nature, 31 October 2012 |
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Valigra L (2012) New Bigelow Lab opens more ocean science possibilities. MaineBiz October 1, 2012 |
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Perkel JM (2012) Single-cell Genomics: Defining Microbiology's Dark Matter. Biotechniques 52:301–303 |
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Heger M. (2012) Focusing on High-Throughput Single-Cell Genomics, Bigelow Looks to Add Sequencing to its Services |
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In dark ocean depths, 'twilight bacteria' at work (2011) |

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Up from the Depths: How Bacteria Capture Carbon in the 'Twilight Zone' (2011) |
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Walker A (2011) Singled out. Nature Reviews 9:485 |

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Curtin C (2011) From the Deep. Genome Technology, May 1 |
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Leslie M (2011) The Power of One. Science 331: 24-26
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Philippidis A (2010) Bigelow Lab Expansion Will Yield Improved Facilities, New Hires for Genomics Center. Genome Technology, September 17 |
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Stepanauskas R (2010) How many bacteria are there in the ocean and what do they all do? |

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Curtin C (2010) Studying the Individual Cell. Genome Technology, March 1 |

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Curtin C (2010) A Unique Snowflake. Genome Technology, March 1 |
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Perkel JM (2009) Surpassing the Law of Averages: How to expose the behaviors of genes,RNA, proteins, and metabolites in single cells. The Scientist 23 |
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Stein RA (2009) Single-Cell Genomics Clarifies Big Picture. Genetic Engineering & Biotechnology News 29 |